Successful delivery derived from vitrified warmed spermatozoa from a patient with nonobstructive azoospermia

One healthy boy was born after transfer of a blastocyst derived from vitrified-warmed spermatozoa from a patient with nonobstructive azoospermia. The spermatozoa were vitrified with the use of Cryotop and Cell Sleeper.

Like Comment


Yuji Endo, B.Sc., Yoshitaka Fujii, Ph.D., Shozo Kurotsuchi, M.D., Ph.D., Hiroaki Motoyama, M.D., Hiroaki Funahashi, Ph.D.

Volume 98, Issue 6, Pages 1423-1427, December 2012



To report the clinical outcomes following intracytoplasmic sperm injection (ICSI) with vitrified sperm from patients with severe male factor infertility.


Retrospective case series.


IVF unit of a medical center.


Three patients with severe oligozoospermia or nonobstructive azoospermia (NOA).


Cryopreservation of limited numbers of spermatozoa using Cryotop and Cell Sleeper as nonbiologic containers.

Main Outcome Measure(s):

Four cycles underwent intracytoplasmic sperm injection (ICSI) with vitrified sperm.


A total of 148 spermatozoa in 18 containers (8.2 sperm per container) were vitrified and 36 of them (5 containers) were warmed. Thirty-three sperm (92%) were retrieved successfully and injected individually into 17 mature oocytes. Fertilization was observed in 12 (71%) oocytes and all zygotes (100%) cleaved. A couple with NOA achieved a singleton pregnancy and concluded with full-term delivery of a healthy boy (2632 g).


A successful delivery was achieved after transfer of a blastocyst derived from vitrified-warmed spermatozoa. A small number of vitrified sperm cells were used for ICSI to fertilize oocytes with predictable timing.

Read the full text at:

Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

No comments yet.