Laura Escrich, Ph.D., Noelia Grau, Ph.D., María José de los Santos, Ph.D., Josep-Lluis Romero, M.D., Antonio Pellicer, M.D., María-José Escribá, Ph.D.
Vol 98, Issue 5, Pages 1147-1151
To evaluate the dynamics of the nuclear maturation (NM) of in vitro-matured (IVM) oocytes and to determine the most favorable duration of meiosis II (MII) arrest in relation to the normal activation response.
University-affiliated infertility clinic.
Donated immature germinal vesicle oocytes (GV).
The GV underwent spontaneous IVM and the dynamics of NM studied by real-time monitoring. IVM oocytes were parthenogenetically activated at different MII arrest points and their response assessed.
Main outcome measure(s):
Moment of GV breakdown; extrusion of the first polar body (1PB); duration of MI and MII arrest; activation rate (AR) and type.
Two GV populations –early (E-IVM: 18.4±2.7hrs) and late (L-IVM: 26.3±3.8hrs; p<0.01) maturing - were defined according to the time required for extrusion of the 1PB. Significantly more E-IVM than L-IVM exhibited a NAR (61.3% vs. 34.6%), but AR were similar (average 88.6%) in both groups. Duration of GV stage differed between the two groups but MI arrest (14.0±0.3hrs) was constant. E-IVM arrested at MII for at least 4.3hrs displayed significantly lower AR and similar NA rates (61.3%) to E-IVM arrested for a shorter time (83.9% vs. 100%). L-IVM displayed a similar AR (80.8%), but lower NA rates than E-IVM (34.6%), regardless of when activation took place. Conclusion(s):
The success of IVM depends on the NM timing rather than on the length of MII arrest.
Read the full text at: http://www.fertstert.org/article/S0015-0282(12)01869-9/fulltext