Aijun Zhang, Ph.D., Bufang Xu, Ph.D., Yijuan Sun, Ph.D., Xiaowei Lu, M.S., Ruihuan Gu, M.S., Ling Wu, M.S., Yun Feng, M.D., Ph.D., Chen Xu, M.D., Ph.D.
Vol 98, Issue 4, Pages 1009-1016
To investigate the distribution patterns of H3K4me3 and H3K27me3 in human oocytes and pre-implantation embryos.
University reproductive medical center.
Patients undergoing in vitro fertilization (IVF) cycles.
Oocytes and embryos were collected from patients undergoing in vitro fertilization cycles.
Main Outcome Measure(s):
The distribution patterns of H3K4me3 and H3K27me3 in oocytes and embryos were analyzed by indirect immunofluorescent staining and scanning confocal microscopy.
H3K4me3 and H3K27me3 signals were detectable at each stage of oocyte and embryonic development. However, only one of the pronuclei showed signal for H3K27me3 in each of the zygotes, whereas H3K4me3 staining was always uniform in all zygotes. The level of H3K4me3 decreased steadily from GV to MII stage, obviously increased from zygote stage to 4-cell stage, and reached the lowest at 8-cell stage. A sharp increase was then observed at blastocyst stage. The level of H3K27me3 slightly changed from GV stage to zygote stage, then decreased steadily and reached the lowest at 8-cell stage, followed by a significant increase at blastocyst stage.
The levels of H3K4me3 and H3K27me3 show dynamic changes during human oocyte maturation and pre-implantation embryonic development. Asymmetric distribution of H3K27me3 exists in human zygote pronuclei, whereas H3K4me3 is always uniform in all of the pronuclei.
Read the full text at: http://www.fertstert.org/article/S0015-0282(12)00687-5/fulltext