A comparison of the effects of N-acetylcysteine and ethyl pyruvate on experimental testicular ischemia-reperfusion injury

This study was intended to compare the effects of NAC and EP on experimental testicular I/R injury. The NAC therapy may be regarded as a relatively more effective treatment than EP.

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Suha Turkmen, M.D., Ahmet Mentese, Ph.D., Ersagun Karaguzel, M.D., Yunus Karaca, M.D., Adem Kucuk, M.D., Aysegul Uzun, Ph.D., Esin Yulug, M.D., Suleyman Turedi, M.D.

Vol 98, Issue 3, Pages 626-631



To compare the effects of NAC and EP on experimental testicular ischemia reperfusion (I/R) injury.


Randomized, controlled, experimental study


University hospital

Animal (s):

Twenty-four mature male Wistar rats

Intervention (s):

Rats were divided into four groups; control, torsion-detorsion (T/D) group, ethyl pyruvate (EP) group and N-acetylcysteine (NAC) group. In the pretreatment of the NAC and EP groups, 20 mg/kg NAC and 50 mg/kg EP were given intraperitoneally 30 min before detorsion.

Main Outcome Measure(s):

Serum ischemia modified albumin (IMA), tissue and serum malondialdehyde (MDA) and myeloperoxidase (MPO) activity levels and histopathological damage scores were then compared.

Result (s):

Ethyl pyruvate and N-acetylcysteine exhibited a protective effect against I/R injury. Of the biochemical parameters evaluated as a result of testicular I/R, only IMA levels were significantly elevated. There was a strong and significant correlation between serum IMA levels and histopathological injury scores, and the increase in serum IMA level exhibited a strong parallel with the rise in histopathological injury. In the EP group, although histopathological injury score was similar to that of the control group, serum IMA levels were significantly elevated.

Conclusion (s):

Both NAC and EP, the effects of which on I/R injury are evaluated here, reduce such injury in testicular T/D. Comparing their effects on IMA levels, NAC may be regarded as a relatively more effective treatment than EP.

Read the full text at: http://www.fertstert.org/article/S0015-0282(12)00588-2/fulltext

Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.