Inhibitory effects of methotrexate on spontaneous motility and cajal-like type of tubal interstitial cells in rabbit oviduct

Methotrexate toxicity causes diminished tubal smooth muscle contractility, probably due to decreased population of t-ICC, which may be new potential targets for a variety of dysfunctional tubal motility diseases.

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Authors

Xiao-Jun Yang, Ph.D., Wei Wei, M.D., Jing Zhao, M.D., Fei-Yun Zheng, M.D.

Vol 98, Issue 1 , Pages 215-221

Abstract

Objective:

To study the adverse biomechanical effects of methotrexate (MTX) on spontaneous tubal motility and on a widely distributed Cajal-like type of tubal interstitial cells (t-ICC) in rabbits. In our previous study, MTX was confirmed to cause acute endosalpingitis, and ultrastructural and steroid receptor damage in rat's endosalpinx in a dose-dependent manner.

Design:

Differences in spontaneous tubal contractions and cellular distribution of t-ICC in isthmus were evaluated in response to MTX.

Setting:

Medical school research laboratory.

Animal(s):

Twenty nonpregnant female New Zealand albino rabbits in estrus stage were divided equally into four groups.

Intervention(s):

Rabbits received IM MTX (1, 5, 10 mg/kg body weight) and controls received physiological saline.

Main Outcome Measure(s):

On day 7, in vitro motility studies measuring spontaneous tubal contractions were performed, and cellular distribution of t-ICC was determined by immunohistochemistry.

Result(s):

Methotrexate produced a concentration-dependent inhibition of spontaneous isthmus contractions (frequency in 5, 10 mg/kg groups, and amplitude in 1, 5, 10 mg/kg MTX groups). It decreased significantly compared with the control group. Meanwhile, MTX at 5, 10 mg/kg decreased the population of c-kit immunoreactive t-ICC significantly.

Conclusion(s):

The decreased t-ICC may contribute to the diminished tubal smooth muscle contractility caused by MTX as observed. Tubal interstitial cells might be new potential targets for a variety of dysfunctional tubal motility diseases.

Read the full text at: http://www.fertstert.org/article/S0015-0282(12)00445-1/fulltext


Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders.