Increased expression of macrophage colony–stimulating factor and its receptor in patients with endometriosis

Patients with endometriosis have higher levels of colony-stimulating factor 1 (CSF-1) and its receptor, C-FMS.

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Authors

Nicole M. Budrys, M.D., M.P.H., Hareesh B. Nair, Ph.D., Ya-Guang Liu, Ph.D., Nameer B. Kirma, Ph.D., Peter A. Binkley, B.S., Shantha Kumar, Ph.D., Robert S. Schenken, M.D., Rajeshwar Rao Tekmal, Ph.D.

Vol 97, Issue 5, Pages 1129-1135.e1

Abstract

Objective:

To investigate the expression and regulation of colony-stimulating factor 1 (CSF-1) and its receptor, C-FMS, in endometriosis.

Design:

In vivo and vitro study.

Setting:

University-based academic medical center.

Patient(s):

Reproductive-age women undergoing surgery for benign conditions.

Intervention(s):

Peritoneal and endometrial tissue samples were obtained.

Main Outcome Measure(s):

CSF-1 and C-FMS expression.

Result(s):

Significantly higher CSF-1 levels were found in peritoneal fluid of patients with endometriosis compared with control subjects. Ectopic endometriotic tissue had 3.5-fold and 1.7-fold increases in CSF-1 and C-FMS expression, respectively, compared with eutopic tissue. Coculture of endometrial cells from either established cell lines or patient samples with peritoneal mesothelial cells (PMCs) led to increased expression of CSF-1 and C-FMS. A higher but nonsignificant increase in levels of C-FMS and CSF-1 was found in cocultures of endometrial epithelial cells from patients with endometriosis compared with those without endometriosis.

Conclusion(s):

Increased CSF-1 levels may contribute to endometriosis lesion formation and progression. Elevation in CSF-1 after coculture of endometrial cells with PMCs suggests that endometrial tissue may be a source of peritoneal CSF-1. Increased C-FMS expression in endometrial cells from women with endometriosis cocultured with PMCs suggests that endometrial tissue involved in lesion formation is highly responsive to CSF-1 signaling.

Read the full text at: http://www.fertstert.org/article/S0015-0282(12)00221-X/fulltext


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Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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