Degradation of actin nucleators affects cortical polarity of aged mouse oocytes

Oocyte polarity was disrupted during aging; we found that the degradation of actin nucleators Arp2/3 complex, JMY, and WAVE2 results in the loss of oocyte polarity in aged mouse oocytes.

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Shao-Chen Sun, Ph.D., Wei-Wei Gao, M.S., Yong-Nan Xu, Ph.D., Yong-Xun Jin, Ph.D., Qing-Ling Wang, Ph.D., Xi-Jun Yin, Ph.D., Xiang-Shun Cui, Ph.D., Nam-Hyung Kim, Ph.D.

Volume 97, Issue 4, Pages 984-990



To investigate the molecular mechanism of mouse oocyte polarity loss during aging.


Experimental study.


Academic basic research laboratory.




Oocytes were collected 16 hours after injection of hCG and cultured in M16 medium for an additional 14 hours with or without caffeine.

Main Outcome Measure(s):

Expression and localizations of actin nucleators actin-related protein 2/3 complex, JMY, and WAVE2 were examined by immunofluorescence staining, and their messenger RNA levels were examined by real-time reverse transcription–polymerase chain reaction.


The protein and messenger RNA levels of actin-related protein 2/3 complex, JMY, and WAVE2 were decreased in aged oocytes, but the levels were normal in caffeine-treated aged oocytes.


Our data indicated that the loss of oocyte polarity may be due to the degradation of actin nucleators in aged oocytes.

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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders.