Degradation of actin nucleators affects cortical polarity of aged mouse oocytes
Oocyte polarity was disrupted during aging; we found that the degradation of actin nucleators Arp2/3 complex, JMY, and WAVE2 results in the loss of oocyte polarity in aged mouse oocytes.
Authors
Shao-Chen Sun, Ph.D., Wei-Wei Gao, M.S., Yong-Nan Xu, Ph.D., Yong-Xun Jin, Ph.D., Qing-Ling Wang, Ph.D., Xi-Jun Yin, Ph.D., Xiang-Shun Cui, Ph.D., Nam-Hyung Kim, Ph.D.
Volume 97, Issue 4, Pages 984-990
Abstract
Objective:
To investigate the molecular mechanism of mouse oocyte polarity loss during aging.
Design:
Experimental study.
Setting:
Academic basic research laboratory.
Animal(s):
Mice.
Intervention(s):
Oocytes were collected 16 hours after injection of hCG and cultured in M16 medium for an additional 14 hours with or without caffeine.
Main Outcome Measure(s):
Expression and localizations of actin nucleators actin-related protein 2/3 complex, JMY, and WAVE2 were examined by immunofluorescence staining, and their messenger RNA levels were examined by real-time reverse transcription–polymerase chain reaction.
Result(s):
The protein and messenger RNA levels of actin-related protein 2/3 complex, JMY, and WAVE2 were decreased in aged oocytes, but the levels were normal in caffeine-treated aged oocytes.
Conclusion(s):
Our data indicated that the loss of oocyte polarity may be due to the degradation of actin nucleators in aged oocytes.
Read the full text at: http://www.fertstert.org/article/S0015-0282(12)00151-3/fulltext
