A tissue-engineered human endometrial stroma that responds to cues for secretory differentiation, decidualization, and menstruation

We report on how a hormonally responsive, engineered human endometrial stroma that can be used to study secretory differentiation, uterine receptivity, and menstruation offers advantages over conventional monolayer cultures.

Upvote 0 Comment


Stacey C. Schutte, Ph.D. and Robert N. Taylor, M.D., Ph.D.

Volume 97, Issue 4, Pages 997-1003



To show the responsiveness of tissue-engineered human endometrial stroma to combinations of hormones that mimic the secretory and menstrual phases of the cycle.


In vitro experimental study.


University uterine biology research laboratory.




Telomerase immortalized human endometrial stromal cells cultured in monolayers (two-dimensional, 2D) or encapsulated in a collagen I hydrogel (three-dimensional, 3D) to create a simplified tissue-engineered stroma were exposed to hormone treatments mimicking early and late secretory phases, decidualization, and steroid withdrawal conditions to recapitulate menstruation.

Main Outcome Measure(s):

Morphologic and biochemical markers of decidualization and collagenase activity.


The 3D tissue can manifest changes in morphology and biochemical markers of decidualization similar to 2D culture and characteristic of endometrial stroma in vivo. Unlike 2D culture, the 3D tissue responded to steroid withdrawal by increased collagenase activity and tissue breakdown.


Three-dimensional tissue-engineered endometrial stroma can mimic secretory and menstrual phases of the cycle and may be useful for studying uterine receptivity and menstruation in a physiological endocrine environment.

Read the full text at: http://www.fertstert.org/article/S0015-0282(12)00127-6/fulltext

Go to the profile of Fertility and Sterility

Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

No comments yet.