Ettie Maman, M.D., Yuval Yung, Ph.D., Alon Kedem, M.D., Gil M. Yerushalmi, M.D., Ph.D., Sarah Konopnicki, B.Sc., Berta Cohen, B.Sc., Jehoshua Dor, M.D., Ariel Hourvitz, M.D.
Volume 97, Issue 3, Pages 592-598
To elucidate the LH receptor (LHR) expression patterns in human granulosa cells (GCs) from antral to preovulatory stages, and to investigate a correlation to oocyte function.
Luteinized preovulatory GCs were obtained from preovulatory follicles aspirated during IVF (≥17 mm). The GCs from small- (
Seventy IVF patients and 20 IVM patients.
Main Outcome Measure(s):
The LHR expression levels in mural and cumulus GCs of different follicular sizes and their correlation to oocyte outcome.
The LHR expression increased with follicle size and was higher in mural GCs compared with cumulus cells. The LHR expression in cumulus GCs from preovulatory follicles was higher in metaphase II (MII) oocytes than in metaphase I or germinal vesicle oocytes (IVF). Unexpectedly, higher expression of LHR in cumulus GCs of MII oocytes correlated with decreased fertilization rates.
The LHR expression in small follicles obtained in IVM suggests a role for hCG administration during IVM procedures. Overexpression of LHR in cumulus GCs of MII oocytes may signal malfunction of oocytes and low fertilization capacity.
Read the full text at: http://www.fertstert.org/article/S0015-0282(11)02911-6/fulltext