Immunization of male mice with B-cell epitopes in transmembrane domains of CatSper1 inhibits fertility

Two B-cell epitopes in transmembrane domains of CatSper1 show immunocontraceptive potential in male mice, and induce high titer of specific antibody, which impairs sperm function and causes sperm agglutination.

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Authors

Honggang Li, Ph.D., Xiaofang Ding, Ph.D., Cuicui Guo, B.A., Huangtao Guan, B.A., Chengliang Xiong, M.D.

Volume 97, Issue 2 , Pages 445-452

Abstract

Objective:

To evaluate the contraceptive ability of two B-cell epitopes in CatSper1.

Design:

In vivo study with mice.

Setting:

University laboratory animal service center.

Animal(s):

BALB/c mice.

Intervention(s):

Two predicted B-cell epitopes in the extracellular part of transmembrane domains and pore region of CatSper1 were synthesized to immunize male mice.

Main Outcome Measure(s):

Fertility, epididymal sperm function, and the presence of specific antibody in immunized males were investigated.

Result(s):

Significant reduction of fertility was observed in mating trial with no evident systemic illness or abnormal mating behavior. Epididymal sperm of epitope-immunized males exhibited impaired ability to fertilize eggs in vitro, and showed sperm agglutination in some animals, while presenting no changes in sperm viability or progressive motility. High titer of antibodies was induced in the sera, and the antibodies’ specificity was confirmed. The binding of the antibodies to epididymal sperm of epitope-immunized males was observed.

Conclusion(s):

CatSper members could be the effective and viable targets for immunocontraception. These two epitopes in CatSper1 share high identity between mouse and human and may be effective for fertility regulation in humans.

Read the full text at: http://www.fertstert.org/article/S0015-0282(11)02819-6/fulltext

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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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