Insufficient histone-3 lysine-9 deacetylation in human oocytes matured in vitro is associated with aberrant meiosis

The increased incidence of aberrant meiosis in in vitro–matured human oocytes correlates with persistence of lysine-9 acetylation of histone-3.

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Authors

Jia Huang, M.D., Tao Li, Ph.D., Chen-Hui Ding, Ph.D., Jan Brosens, Ph.D., Can-Quan Zhou, M.D., Hui-Hui Wang, M.D., Yan-Wen Xu, Ph.D.

Volume 97, Issue 1 , Pages 178-184.e3

Abstract

Objective:

To characterize histone acetylation during meiosis in human oocytes matured in vitro or in vivo.

Design:

Experimental study.

Setting:

University reproductive medical center.

Patient(s):

Patients undergoing routine intracytoplasmic sperm injection (ICSI) cycles.

Intervention(s):

Immature and mature oocytes were collected from patients undergoing intracytoplasmic sperm injection.

Main Outcome Measure(s):

Immunohistochemical assessment of the levels of acetylated lysine-9 of histone-3 (H3K9) and lysine-12 of histone-4 (H4K12) combined with spindle and chromosome configurations in in vitro– and in vivo–matured human oocytes. Transcript levels of histone deacetylases (HDACs) 1 and 2 were measured by single-cell quantitative polymerase chain reaction.

Result(s):

Acetylation of H3K9 and H4K12 decreased during human oocyte maturation. Residual H3K9 acetylation was found in 37.7% of oocytes matured in vitro, compared with 11.8% in oocytes matured in vivo. Abnormal metaphase spindle was more frequent in oocytes with residual histone acetylation than without (51.6% vs. 25.4%, respectively). Treatment with the HDAC inhibitor trichostatin A increased the incidence of an abnormal metaphase but had no adverse effect on maturation efficiency. Furthermore, expression of HDAC1 transcripts was significantly lower in oocytes matured in vitro versus in vivo.

Conclusion(s):

Reduced HDAC1 expression and insufficient histone deacetylation are associated with metaphase defects in human oocytes matured in vitro.

Read the full text at: http://www.fertstert.org/article/S0015-0282(11)02708-7/fulltext

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Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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