Relationships between age of 25K men attending infertility clinics and SCSA defined sperm DNA and chromatin integrity
Sperm DNA fragmentation increases with age (20 80 years) in infertility patients at about the same rate as in healthy men without reproductive issues.
VOLUME 114, ISSUE 2, P311-320
Authors:
Donald P. Evenson, Ph.D., Gemechis Djira, Ph.D., Kay Kasperson, B.S., Jennifer Christianson, A.A.S.
Abstract:
Objective
To determine relationships between age of men with potential male factor infertility and sperm chromatin structure assay (SCSA) measures of sperm DNA fragmentation (SDF) and high DNA stainable sperm (HDS), and to compare these data with those obtained from healthy donor men without reproductive issues.
Design
Retrospective study.
Setting
Infertility clinics and diagnostic laboratory.
Patients
A total of 25,445 men attending infertility clinics. Donors were 87 men working at Lawrence Livermore National Laboratory.
Intervention
None.
Main Outcome Measures
SCSA measures (% DNA fragmentation index (DFI), X DFI, SD DFI, and %HDS) of men aged 21−80 years.
Results
In the study population, advancing paternal age was associated with increased sperm DNA fragmentation (SDF) scored as increased percentage of sperm in semen ejaculates with measurable DNA strand breaks (%DFI). The slope of increase in %DFI prior to age 41.6 years was 0.39, which increased after age 41.6 to more than double at a slope of 0.86. These changes in DNA/chromatin in more than 25,000 aging men attending infertility clinics are similar to those seen over the same age span (20−80 years) in 87 nonpatient, healthy men without reproductive issues. For the age group 20−50 years, there was no major significant difference in %DFI between patients and donor men. According to a logistic regression model, the estimated probability is that, for example, a 40-year-old and a 50-year-old man have a 20% and 40% chance, respectively, to have a pathological DFI ≥25% by age factor alone. The condensation of sperm chromatin in patients increased with age in a linear fashion, from a mean of 12.2 %HDS at age 20−25 to a mean of 7.9 %HDS at age 60−65. Patients had a greater %HDS than donors across all ages.
Conclusions
The great heterogeneity of both DFI and HDS values at a specific age prevents the automatic translation of age into an index of DNA fragmentation. However, it reinforces the idea that both DFI and HDS evaluation can play a role in detecting potential male infertility in cases that are not resolved by routine testing and in cases of multiple miscarriages. DFI and HDS data can help clinicians to predict a man’s fertility potential, to consider corrective therapeutic approaches, as well as to assess the risk to the offspring’s health.
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