Characterization of exosomes in peritoneal fluid of endometriosis patients

In exosomes isolated directly from the peritoneal fluid of women, the exosome protein cargo differed between endometriosis patients and controls, and the proteins identified could serve as biomarkers of endometriosis.

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Volume 113, Issue 2, Pages 364–373.e2

Authors:

Hannah M. Nazri, M.D., Maria Imran, B.Sc., Roman Fischer, Ph.D., Raphael Heilig, Ph.D., Sanjiv Manek, M.D., Rebecca A. Dragovic, Ph.D., Benedikt M. Kessler, Ph.D., Krina T. Zondervan, Ph.D., Thomas T. Tapmeier, Ph.D., Christian M. Becker, M.D.

Abstract:

Objective

To demonstrate the feasibility of studying exosomes directly from peritoneal fluid, we isolated exosomes from endometriosis patient samples and from controls, and characterized their cargo.

Design

Case-control experimental study.

Setting

Academic clinical center.

Patient (s)

Women with and without endometriosis who underwent laparoscopic surgery (n = 28 in total).

Intervention (s)

None.

Main Outcome Measure (s)

Concentration of exosomes within peritoneal fluid and protein content of the isolated exosomes.

Result (s)

Peritoneal fluid samples were pooled according to the cycle phase and disease stage to form six experimental groups, from which the exosomes were isolated. Exosomes were successfully isolated from peritoneal fluid in all the study groups. The concentration varied with cycle phase and disease stage. Proteomic analysis showed specific proteins in the exosomes derived from endometriosis patients that were absent in the controls. Five proteins were found exclusively in the endometriosis groups: PRDX1, H2A type 2-C, ANXA2, ITIH4, and the tubulin α-chain.

Conclusion (s)

Exosomes are present in peritoneal fluid. The characterization of endometriosis-specific exosomes opens up new avenues for the diagnosis and investigation of endometriosis.


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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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