Oocyte meiotic spindle morphology is a predictive marker of blastocyst ploidy—a prospective cohort study

Oocyte meiotic spindle morphology is a predictive marker of blastocyst ploidy. Normal spindle morphology is positively associated with fertilization, blastocyst quality, and euploidy.

Volume 113, Issue 1, Pages 105–113.e1


Liza Tilia, B.Sc., Michael Chapman, M.D., Suha Kilani, Ph.D., Simon Cooke, Ph.D., Christos Venetis, Ph.D.



To evaluate oocyte meiotic spindle (OMS) morphology at intracytoplasmic sperm injection (ICSI) as a predictor of blastocyst ploidy and whether OMS morphology could aid standard morphology-based blastocyst selection.


Prospective cohort study.


In vitro fertilization clinic.


Patients undergoing ICSI cycles with an intention to perform preimplantation genetic testing for aneuploidy (PGT-A) from October 2014 to December 2017.


The OMS was visualized with the use of polarized light microscopy at the time of ICSI and the morphology classified as normal, dysmorphic, translucent, not visible, or in telophase. Blastocyst biopsy for PGT-A was performed on embryos with suitable development.

Main Outcome Measure(s)

The association of OMS morphology with the resulting blastocyst ploidy was evaluated on an “intention-to-treat” (ITT) and an “as-treated analysis” (ATA) basis.


The morphology of 2,056 OMSs were classified. A strong association of OMS morphology with fertilization, cleavage to at least 6 cells on day 3, and good/top-quality blastocyst formation was present. Normal OMS was positively associated with blastocyst euploidy compared with all other OMS types combined, per either ITT or ATA. Even after controlling for female age, blastocyst quality, and developmental stage, the presence of a normal OMS was strongly associated with the probability of blastocyst euploidy.


OMS morphology is a predictive marker of blastocyst ploidy and can potentially aid standard morphology-based blastocyst selection.

Read the full text here.

Please sign in or register for FREE

Your Fertility and Sterility Dialog login information is not the same as your ASRM or EES credentials. Users must create a separate account to comment or interact on the Dialog.

Go to the profile of Samuel Santos-Ribeiro
about 3 years ago

Very interesting results from the group of Tilia et al, expanding on their previous work from 2016. In one shot, the authors present interesting hypotheses to justify the frequent occurrence of aneuploid and mosaic embryos in ART. Pending further validation, we may soon have a more robust manner to assess oocyte competence.