Comparison of microfluid sperm sorting chip and density gradient methods for use in intrauterine insemination cycles

The microfluid sperm sorting method significantly increased the ongoing pregnancy rates compared with the density gradient group in intrauterine insemination cycles.

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Volume 112, Issue 5, Pages 842–848.e1


Funda Gode, M.D., Taylan Bodur, M.D., Fatma Gunturkun, Ph.D., Ali Sami Gurbuz, M.D., Burcu Tamer, M.Sc., Ibrahim Pala, M.Sc., Ahmet Zeki Isik, M.D.



To compare the effect of microfluiding sperm sorting chip and density gradient methods on ongoing pregnancy rates (PRs) of patients undergoing IUI.


Retrospective cohort study.


Hospital IVF unit.


Couples with infertility undergoing IUI cycles between 2017 and 2018.


Not applicable.

Main Outcome Measure(s)

Ongoing PRs.


A total of 265 patients were included in the study. Microfluid sperm sorting and density gradient were used to prepare sperm in 133 and 132 patients, respectively. Baseline spermiogram parameters, including volume, concentration, motility, and morphology, were similar between the two groups. Total motile sperm count was lower in the microfluiding sperm sorting group at baseline (35.96 ± 37.69 vs. 70.66 ± 61.65). After sperm preparation sperm motility was higher in the microfluid group (96.34 ± 7.29 vs. 84.42 ± 10.87). Pregnancy rates were 18.04% in the microfluid group and 15.15% in the density gradient group, and ongoing PRs were 15.03% and 9.09%, respectively. After using multivariable logistic regression and controling for confounding factors, there was a significant increase in ongoing PRs in the microfluid sperm sorting group. The adjusted odds ratio for ongoing pregnancy in the microfluid group compared with the density gradient group was 3.49 (95% confidence interval 1.12–10.89).


The microfluid sperm sorting method significantly increased the ongoing PRs compared with the density gradient group in IUI cycles.

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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. 


Go to the profile of Samuel Santos-Ribeiro
about 2 years ago

Thank you for showing us this very interesting study! I have a question for the authors: there seems to be an imbalance among the groups in terms of the number of preceding cycles. One common finding when IUI is performed using ovarian stimulation is to increase the dose of gonadotropins in subsequent cycles if only one follicle developed. I noticed that the authors have information on the duration of stimulation and on the late-follicular endometrial thickness, so I have to wonder whether they could by any chance have the data to compare the groups regarding a) the total dose of stimulation performed b) the number of follicles larger than 14 mm visible on the day of ovulation triggering and c) multiple pregnancy rates. As physicians, we know that these are very important variables to account for in retrospective studies assessing IUI outcomes, especially by including the number of large follicles available for triggering in the regression models.