Pregnancy outcomes from more than 1,800 in vitro fertilization cycles with the use of 24-chromosome single-nucleotide polymorphism–based preimplantation genetic testing for aneuploidy

Selective transfer of embryos determined to be euploid by 24-chromosome single-nucleotide polymorphism–based preimplantation genetic testing for aneuplody demonstrates excellent in vitro fertilization outcomes.

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Volume 110, Issue 1, Pages 113–121

Authors:

Alexander L. Simon, B.S., Michelle Kiehl, M.Sc., Erin Fischer, B.S., J. Glenn Proctor, M.H.A., Mark R. Bush, M.D., Carolyn Givens, M.D., Matthew Rabinowitz, Ph.D., Zachary P. Demko, Ph.D.

Abstract:

Objective

To measure in vitro fertilization (IVF) outcomes following 24-chromosome single‒nucleotide-polymorphism (SNP)–based preimplantation genetic testing for aneuploidy (PGT-A) and euploid embryo transfer.

Design

Retrospective.

Setting

Fertility clinics and laboratory.

Patient(s)

Women 20–46 years of age undergoing IVF treatment.

Intervention(s)

Twenty-four-chromosome SNP-based PGT-A of day 5/6 embryo biopsies.

Main Outcome Measure(s)

Maternal age–stratified implantation, clinical pregnancy, and live birth rates per embryo transfer; miscarriage rates; and number of embryo transfers per patient needed to achieve a live birth.

Result(s)

An implantation rate of 69.9%, clinical pregnancy rate per transfer of 70.6%, and live birth rate per transfer of 64.5% were observed in 1,621 nondonor frozen cycles with the use of SNP-based PGT-A. In addition, SNP-based PGT-A outcomes, when measured per cycle with transfer, remained relatively constant across all maternal ages; when measured per cycle initiated, they decreased as maternal age increased. Miscarriage rates were ∼5% in women ≤40 years old. No statistically significant differences in pregnancy outcomes were found for single-embryo transfers (SET) versus double-embryo transfers with SNP-based PGT-A. On average, 1.38 embryo transfers per patient were needed to achieve a live birth in nondonor cycles.

Conclusion(s)

Our findings that SNP-based PGT-A can mitigate the negative effects of maternal age on IVF outcomes in cycles with transfer, and that pregnancy outcomes from SET cycles are not significantly different from those of double-embryo transfer cycles, support the use of SET when transfers are combined with SNP-based PGT-A.


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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders.