Using microscope for onco-testicular sperm extraction for bilateral testis tumors

Use of the operating microscope for onco-testicular sperm extraction may allow for more precise dissection for fertility preservation

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Volume 109, Issue 4, Page 745


Robert Carrasquillo, M.D., Luis Felipe Sávio, M.D., Vivek Venkatramani, M.D., Dipen Parekh, M.D., Ranjith Ramasamy, M.D.



To demonstrate a step-by-step approach to the use of the operating microscope for onco-testicular sperm extraction.


Video presentation.


University hospital.


A 34-year-old man (status post right orchiectomy at another institution for pT3 pure seminoma with negative preoperative tumor markers) was referred for contralateral orchiectomy for multifocal left testis mass and fertility preservation. A postoperative semen analysis for attempted cryopreservation of ejaculated semen identified azoospermia.


Left radical orchiectomy, left microsurgical onco-testicular sperm extraction (TESE).

Main Outcome Measure(s)

Intraoperative technique with commentary highlighting tips for successful fertility preservation via microsurgical onco-TESE. Discussion of alternatives.


This video provides a step-by-step guide to microsurgical onco-TESE coordinated with radical orchiectomy for testis cancer as a means of fertility preservation in an azoospermic patient. Preoperative imaging with scrotal ultrasound can serve as a useful guide for targeting microdissection to areas of normal testicular parenchyma for extraction of seminiferous tubules likely to host normal spermatogenesis. This patient had successful recovery and cryopreservation of abundant testicular sperm following targeted ex-vivo testicular microdissection.


Microsurgical onco-TESE may be offered to azoospermic patients when undergoing orchiectomy for testis cancer. Use of preoperative imaging and the surgical microscope guide surgical dissection and optimize sperm recovery.

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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders.