Fertility rescue and ovarian follicle growth promotion by bone marrow stem cell infusion
Human bone marrow–derived stem cell infusion sup- plied an optimal environment to promote follicle growth and fertility rescue in chemotherapy-damaged mouse ovaries and in human ovarian biopsies from poor-responder women.
Volume 109, Issue 5, Pages 908–918.e2
Sonia Herraiz, Ph.D., Anna Buigues, B.Sc., César Díaz-García, M.D., Mónica Romeu, M.D., Susana Martínez, M.D., Inés Gómez-Seguí, M.D., Carlos Simón, M.D., Aaron J. Hsueh, Ph.D., Antonio Pellicer, M.D.
To assess if infusion of human bone marrow–derived stem cells (BMDSCs) could promote follicle development in patients with impaired ovarian functions.
University research laboratories.
Immunodeficient NOD/SCID female mice.
Human BMDSCs were injected into mice with chemotherapy-induced ovarian damage and into immunodeficient mice xenografted with human cortex from poor-responder patients (PRs).
Main Outcome Measure(s)
Follicle development, ovulation, and offspring. Apoptosis, proliferation, and vascularization were evaluated in mouse and human ovarian stroma.
Fertility rescue and spontaneous pregnancies were achieved in mice ovaries mimicking PRs and ovarian insufficiency, induced by chemotherapy, after BMDSC infusion. Furthermore, BMDSC treatment resulted in production of higher numbers of preovulatory follicles, metaphase II oocytes, 2-cell embryos, and healthy pups. Stem cells promoted ovarian vascularization and cell proliferation, along with reduced apoptosis. In xenografted human ovarian tissues from PRs, infusion of BMDSCs and their CD133+ fraction led to their engraftment close to follicles, resulting in promotion of follicular growth, increases in E2 secretion, and enhanced local vascularization.
Our results raised the possibility that promoting ovarian angiogenesis by BMDSC infusion could be an alternative approach to improve follicular development in women with impaired ovarian function.