Next generation sequencing for preimplantation genetic screening improves pregnancy outcomes compared with array comparative genomic hybridization in single thawed euploid embryo transfer cycles

Next generation sequencing for preimplantation genetic screening in single thawed euploid ET cycles improves ongoing pregnancy rates compared with array comparative genomic hybridization.

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Volume 109, Issue 4, Pages 627–632

Authors:

Jenna Friedenthal, M.D., Susan M. Maxwell, M.D., Santiago Munné, Ph.D., Yael Kramer, M.S., David H. McCulloh, Ph.D., Caroline McCaffrey, Ph.D., James A. Grifo, M.D., Ph.D.

Abstract:

Objective

To evaluate whether the use of next generation sequencing (NGS) for preimplantation genetic screening (PGS) in single thawed euploid embryo transfer (STEET) cycles improves pregnancy outcomes compared with array comparative genomic hybridization (aCGH).

Design

Retrospective cohort study.

Setting

Single university-based fertility center.

Patient(s)

A total of 916 STEET cycles from January 2014 to December 2016 were identified. Cases included 548 STEET cycles using NGS for PGS and controls included 368 STEET cycles using aCGH for PGS.

Intervention(s)

Patients having a STEET after undergoing IVF and PGS with either NGS or aCGH.

Main Outcome Measure(s)

Primary outcomes were implantation rate, ongoing pregnancy/live birth rate (OP/LBR), biochemical pregnancy rate (PR), and spontaneous abortion (SAB) rate.

Result(s)

The implantation rate was significantly higher in the NGS group compared with the aCGH group (71.6% vs. 64.6%). The OP/LBR was also significantly higher in the NGS group (62% vs. 54.4%), and there were significantly more biochemical pregnancies in the aCGH group compared with the NGS group (15.1% vs. 8.7%). After adjustment for confounding variables with a multiple logistic regression analysis, OP/LBR remained significantly higher in the NGS group. The SAB rate was not significantly different in the NGS group compared with the aCGH group (12.4% vs. 12.7%).

Conclusion(s)

Preimplantation genetic screening using NGS significantly improves pregnancy outcomes versus PGS using aCGH in STEET cycles. Next-generation sequencing has the ability to identify and screen for embryos with reduced viability such as mosaic embryos and those with partial aneuploidies or triploidy. Pregnancy outcomes with NGS may be improved due to the exclusion of these abnormal embryos.


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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

2 Comments

Go to the profile of Mar Estevan Guimerà
Mar Estevan Guimerà over 2 years ago

Hello, I was ask to explain your article in class for my master's degree in asisted reproduction and I cannot understand the Table 3.

Why do you adjust each parameter with different variables? 

Sorry for the annoyances!

Go to the profile of Micah J Hill
Micah J Hill over 2 years ago

Thank you for the comment Mar.  No comments or questions on the Fertility and Sterility Dialog  are an annoyance!  We look forward to the author interacting with you.