Female age, serum antimüllerian hormone level, and number of oocytes affect the rate and number of euploid blastocysts in in vitro fertilization/intracytoplasmic sperm injection cycles

A positive age-independent relationship between antimullerian hormone and the rate of euploid blastocysts has been found. This confirms that the measurement of ovarian reserve has high relevance when counselling infertile patients.

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Volume 108, Issue 5, Pages 777–783.e2

Authors:

Antonio La Marca, M.D., Ph.D., Maria Giulia Minasi, M.Sc., Giovanna Sighinolfi, M.D., Pierfrancesco Greco, M.D., Cindy Argento, M.D., Valentina Grisendi, M.D., Francesco Fiorentino, Ph.D., Ermanno Greco, M.D.

Objective

To study the relative role of female age and ovarian reserve, measured through serum antimüllerian hormone (AMH) in determining the rate and number of euploid blastocysts in in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycles.

Design

Retrospective analysis of cycles performed in 2014–2015.

Setting

Tertiary referral IVF center.

Patient(s)

A total of 578 infertile couples undergoing IVF/ICSI and preimplantation genetic screening (PGS) analysis.

Interventions(s)

All embryos were cultured and biopsied at the blastocyst stage. The method involved whole-genome amplification followed by array comparative genome hybridization. Serum AMH was measured by means of the modified Beckman Coulter AMH Gen II assay.

Main Outcome Measures

The rate and number of euploid blastocysts and their correlation with ovarian reserve and response to stimulation.

Result(s)

The mean (±SD) age of patients was 37.6 ± 4.1 years, and the mean number of blastocysts per patient was 3.1 ± 2. The total number of blastocysts available to the analysis was 1,814, and 36% of them were euploid after PGS. Age and serum AMH were significantly and independently related to the rate of euploid blastocysts available for patients. As an effect of the cohort size, the number of mature oocytes positively affected the total number of euploid blastocysts per patient.

Conclusion(s)

A strong positive age-independent relationship between AMH level and the rate of euploid blastocysts was found. This confirms that the measurement of ovarian reserve by means of AMH has high relevance when counseling infertile patients.


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Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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