Effects of high progesterone level on the day of human chorionic gonadotrophin administration in in-vitro fertilization cycles on epigenetic modification of endometrium in peri-implantation period

High progesterone level on human chorionic gonadotropin administration day affects the epigeneticmodification in endometrium during the peri-implantation period. The epigenetic markers’ expression was mainly correlated with progesterone level.

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Volume 108, Issue 2, Pages 269–276.e1

Authors:

Yujing Xiong, M.Phil., Jianzhang Wang, M.Phil., Liu Liu, M.D., Xiaoyan Chen, M.Phil., Hui Xu, Ph.D., Tin Chiu Li, M.D., Ph.D., Chi Chiu Wang, Ph.D., Songying Zhang, M.D., Ph.D.

Abstract:

Objective

To investigate the effects of high P level on the day of hCG administration in IVF cycles on epigenetic modifications of endometrium in the peri-implantation period.

Design

Retrospective study.

Setting

University assisted reproductive unit.

Patient(s)

Forty patients were recruited: 20 women with high P levels were compared with 20 women with normal P levels.

Intervention(s)

Endometrial tissues were collected 7 days after hCG administration in women with normal or high P levels on the day of hCG administration. Immunohistochemical staining of DNA methylation (5-methylcytosine, 5-mC), histone methylation (H3K4me2/3, H3K9me2, H3K27me3), and histone acetylation (H3K4ac, H3K9ac) was performed.

Main Outcome Measure(s)

A semiquantitative analysis was performed in the luminal epithelium, glandular epithelium, and stroma, separately. Correlations between P, estrogen levels, and epigenetic markers were compared.

Result(s)

In luminal epithelium, the expression of H3K9me2 in the high P group was significantly higher than that in the normal P group. In glandular epithelium, the expression of 5-mC, H3K9me2, and H3K9ac in the high P group was significantly higher than that in the normal P group. In stroma, the expression of H3K27me3 in the high P group was significantly higher than that in the normal P group. Expression of 5-mC in glandular epithelium, H3k9me2 in glandular and luminal epithelium, and H3K27me3 in stroma was significantly correlated with P levels. H3k9me2 in glandular epithelium was significantly correlated with estrogen levels.

Conclusion(s)

High P level on the day of hCG administration in IVF cycles affects epigenetic modifications in three compartments of endometrium in the peri-implantation period. The epigenetic markers' expression was mainly correlated with P level. The altered epigenetic modification status in the endometrium may disrupt the endometrial receptivity in women with high P on the day of hCG administration.


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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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