Evaluation of amnion in creation of neovagina in women with Mayer-Rokitansky-Kuster-Hauser syndrome

A retrospective study was done in 50 women who underwent amnion neovaginoplasty, and it was found to have a successful outcome in terms of anatomic, physiologic, and functional results.

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Volume 108, Issue 2, Pages 341–345


Richa Vatsa, M.D., Juhi Bharti, M.D., Kallol Kumar Roy, M.D., Sunesh Kumar, M.D., Jai Bhagwan Sharma, M.D., Neeta Singh, M.D., Seema Singhal, M.D., Jyoti Meena, M.D.



To assess the outcome of amnion vaginoplasty in cases of vaginal agenesis due to Mayer-Rokitansky-Kuster-Hauser (MRKH) syndrome managed at the authors' institution.


Retrospective study.


Tertiary care hospital.


Fifty women with MRKH who underwent neovaginoplasty.


Modified McIndoe's vaginoplasty was done in all the patients, using human amnion graft.

Main Outcome Measure(s)

Functional status assessed by Female Sexual Function Index, anatomic status (length and width of neovagina), and epithelialization of vagina.


Mean (±SD) vaginal length after surgery was 8.2 ± 1 cm. Mean vaginal length at 6-month follow-up in sexually active patients was significantly longer as compared with the patients who were not sexually active after surgery (8.4 ± 1.04 cm vs. 6.6 ± 2.4 cm). Mean Female Sexual Function Index score was 30.8 ± 2.1. Vaginal biopsy showed complete epithelialization of vaginal mucosa.


In a developing nation like India, McIndoe's method with amnion graft seems to be a promising option owing to its low cost, easy availability, and safety, ease of the procedure not requiring any special instrument, physiologic outcome with respect to epithelialization of the vagina without hair growth, and satisfying functional outcome.

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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders.