Expression of ciliated bronchial epithelium 1 during human spermatogenesis

Ciliated bronchial epithelium 1 is transiently localized at microtubule-based structures in elongating sperma- tids and absent in spermatozoa, indicating a crucial role in spermatogenesis but not in the maintenance of spermatozoa.

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Volume 108, Issue 1, Pages 47–54

Authors:

Christiane Pleuger, M.Sc., Daniela Fietz, Dr.Med.Vet., Katja Hartmann, Dr.Med.Vet., Hans-Christian Schuppe, Dr.Med., Wolfgang Weidner, Dr.Med., Sabine Kliesch, Dr.Med., Mark Baker, Ph.D., Moira K. O'Bryan, Ph.D., Martin Bergmann, Dr.Rer.Medic.

Abstract:

Objective

To define the precise cellular localization of ciliated bronchial epithelium 1 (CBE1) in the human testis and test its relationship to impaired spermatogenesis.

Design

Gene expression analysis, and histologic and immunohistochemical evaluation.

Setting

University research laboratories and andrologic outpatient clinic.

Patient(s)

Forty-three human testicular biopsies: 12 biopsies showing normal spermatogenesis (NSP), 8 with maturation arrest at level of spermatocytes (STA), 8 with maturation arrest at level of spermatids (SDA), 4 with scattered elongating spermatids, and 12 with Sertoli cell-only syndrome, with an additional 5 semen samples from healthy donors.

Intervention(s)

None.

Main Outcome Measure(s)

Evaluation of CBE1 expression in normal as well as impaired spermatogenesis on mRNA (quantitative reverse-transcription polymerase chain reaction and in situ hybridization) and protein level (immunohistochemistry, Western blot analysis).

Result(s)

In normal spermatogenesis, CBE1 mRNA was expressed in late pachytene spermatocytes, and the protein was localized within the flagellum of elongating spermatids from stage V up to the spermiation in stage II. Immunoelectron microscopy showed CBE1 clearly associated with microtubules at the manchette, the head-tail coupling apparatus, and the flagellum, but the protein was absent in spermatozoa. Compared with normal spermatogenesis, CBE1 mRNA was statistically significantly reduced in samples with a maturation arrest at the level of round spermatids and primary spermatocytes, and was absent in samples showing Sertoli cell-only syndrome. CBE1 protein was completely missing in SDA samples showing few elongating spermatids.

Conclusion(s)

Our data strongly suggest an influence of CBE1 in ciliogenesis in spermatids due to the localization at the microtubules of the elongating spermatids, indicating a role in the intramanchette and/or intraflagellar transport mechanism. The absence of CBE1 in spermatozoa suggests that CBE1 is important for the spermatid development but not for the maintenance of mature spermatozoa as a component of the flagellum.


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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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