Does cryopreservation of sperm effect fertilization in non-obstructive azoospermia or cryptozoospermia? Does cryopreservation of sperm affect fertilization in nonobstructive azoospermia or cryptozoospermia?

In patients with cryptozoospermia, intracytoplasmic sperm injection outcomes are superior in fresh compared with frozen ejaculated sperm, whereas in nonobstructive azoospermia fresh and frozen testicular sperm yielded similar results.

Volume 107, Issue 5, Pages 1148–1152


Natali Schachter-safrai, M.D., Gilad Karavani, M.D., Eliahu Levitas, M.D., Michael Friger, Ph.D., Atif Zeadna, M.D., Eitan Lunenfeld, M.D., Iris Har-Vardi, Ph.D.


In cases of cryptozoospermia, frozen-thawed ejaculated sperm is inferior to fresh ejaculated sperm in fertilization rates. However, in nonobstructive azoospermia, no major differences were found between fresh and frozen-thawed testicular sperm. Therefore, uncoupled TESE/oocyte pick-up (OPU) should be considered in NOA cases to prevent possible unnecessary ovarian stimulation and OPU when no sperm cells are detected.

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