The most well kept secret, embryo culture media: a smart reveal from an expert


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Volume 107, Issue 4, Page 910


Marcos Meseguer, Ph.D., Antonio Pellicer, M.D.


The introduction of in vitro fertilization (IVF) treatments was only possible when all those investigations that took place in tissue culture were adapted for human embryos. But human embryo culture is very different from other cell culture, because somatic cells are surrounded by the extracellular fluid rich in nutrients whereas embryos are simply bounded by a very low amount of uterine fluid that reflects location and chronologic changes (1). Since the beginning of assisted reproductive technology (ART) in the 1980s, media conception, configuration, and composition may have changed dramatically, and we may consider that a portion of the improved success in IVF programs came along with enhanced formulations and detailed user protocols (2).

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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility┬« is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. 


Go to the profile of Carlos Gilberto Almodin
almost 5 years ago
As far as I know the vast majority of commercial culture media possess components which are very similar. The main concern I have today is the lack of consensus as regards of single or sequential media, and the difference in oxygen in incubators. In Brazil, we live a stage in which all embryologists are measuring pH of the medium inside the incubator without a predefined protocol. I would appreciate if someone could tell me if they have a specific protocol to be used in the incubator to measure medium pH, and if Marcos could clarify what exactaly he meant by "incorrect set up in culture media in the lab". Gilberto Almodin, MD, PhD.