Optimal culture conditions are critical for efficient expansion of human testicular somatic and germ cells in vitro

Improved human testicular germ cell survival in DMEM-F12 medium is attributed to limited somatic cell growth compared with commonly used Stempro- 34 medium. The adoption of DMEM-F12 medium for human spermatogonial stem cell culture may improve in vitro human spermatogonial stem cell proliferation.

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Volume 107, Issue 3, Pages 595–605

Authors:

Itai Gat, M.D., Leila Maghen, M.Sc., Melissa Filice, M.Sc., Brandon Wyse, M.Sc., Khaled Zohni, M.D., Keith Jarvi, M.D., Kirk C. Lo, M.D., Andrée Gauthier Fisher, Ph.D., Clifford Librach, M.D.

Abstract:

Use of DMEM-F12 reduces TSC proliferation while preserving their unique characteristics, leading to improved germ cell aggregates formation compared with StemPro-34, the standard basal medium used in the majority of previous reports.


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Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders.