Morphologic grading of euploid blastocysts influences implantation and ongoing pregnancy rates

Blastocyst morphology, particularly inner cell mass grade, is a strong predictor of ongoing pregnancy rate per euploid embryo. It should be used to help select among euploid blastocysts.
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Volume 107, Issue 3, Pages 664–670

Authors:

Mohamad Irani, M.D., David Reichman, M.D., Alex Robles, M.D., Alexis Melnick, M.D., Owen Davis, M.D., Nikica Zaninovic, Ph.D., Kangpu Xu, Ph.D., Zev Rosenwaks, M.D.

Abstract:

Objective

To determine whether blastocyst grading can predict pregnancy outcomes in the frozen-thawed embryo transfer (FET) of euploid blastocysts.

Design

Retrospective cohort study.

Setting

Academic medical center.

Patient(s)

Women who underwent FET of euploid embryo(s) between January 2013 and December 2015, with blastocysts were divided into four groups based on their morphologic grading before cryopreservation: excellent (≥3AA), good (3–6AB, 3–6BA, 1–2AA), average (3–6BB, 3–6AC, 3–6CA, 1–2AB, 1–2BA), and poor (1–6BC, 1–6CB, 1–6CC, 1–2BB).

Intervention(s)

FET.

Main Outcomes Measure(s)

Ongoing pregnancy rate (OPR).

Result(s)

A total of 417 FET cycles (477 embryos) were included. Excellent-quality embryos (n = 38) yielded a statistically significantly higher OPR than poor-quality embryos (n = 106) (84.2% vs. 35.8%; adjusted odds ratio 11.0; 95% confidence interval, 3.8–32.1) and average-quality embryos (n = 197) (84.2% vs. 55.8%; adjusted odds ratio 4.8; 95% confidence interval, 1.7–13.3). Good-quality embryos (n = 76) were associated with a statistically significantly higher OPR than poor-quality embryos (61.8% vs. 35.8%). These odds ratios were adjusted for patient's age, body mass index, number of transferred embryos, type of frozen cycle, peak endometrial thickness, day of trophectoderm biopsy (5 or 6), and total number of euploid embryos for each patient. An inner cell mass grade of A yielded a statistically significantly higher OPR than ICM grade C (76.2% vs. 13.5%) or grade B (76.2% vs. 53.6%) after controlling for all confounders.

Conclusion(s)

Contrary to prior published studies, the current data suggest that blastocyst morphologic grading and particularly inner cell mass grade is a useful predictor of OPR per euploid embryo. Morphologic grading should be used to help in the selection among euploid blastocysts.


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