Ferroportin mRNA is down-regulated in granulosa and cervical cells from infertile women
Iron metabolism (as evidenced by the proxy marker ferroportin mRNA in granulosa and cervical cells and by circulating hepcidin) is altered in infertile women.
Volume 107, Issue 1, Pages 236-242
José Maria Moreno-Navarrete, Ph.D., Eva López-Navarro, Ph.D., Luz Candenas, Ph.D., Francisco Pinto, Ph.D., Francisco J. Ortega, Ph.D., Mònica Sabater-Masdeu, M.Sc., Manuel Fernández-Sánchez, M.D., Ph.D., Victor Blasco, M.Sc., Antonio Romero-Ruiz, Ph.D., Marina Fontán, M.Sc., Wifredo Ricart, M.D., Ph.D., Manuel Tena-Sempere, M.D., Ph.D., José M. Fernández-Real, M.D., Ph.D.
To explore the relationship between iron and infertility by investigating iron-related gene expression in granulosa and uterine cervical cells.
Two tertiary hospitals.
Two independent cohorts of fertile (n = 18 and n = 17) and infertile (n = 31 and n = 35) women.
In vitro fertilization.
Main Outcome Measure(s)
Gene expression levels of ferritin light chain (FTL), ferritin heavy chain (FTH), transferrin receptor (TFRC), and ferroportin (SLC40A1) mRNA were analyzed in granulosa and cervical cells.
In the first cohort, fertile and infertile women were similar in body mass index. Ferroportin mRNA levels were decreased in granulosa cells from infertile women in parallel with increased serum hepcidin levels. A positive association between ferroportin and TFRC mRNA, a gene associated with intracellular iron deficiency, was observed only in granulosa cells from fertile women. The major findings were replicated in a second independent cohort.
Ferroportin mRNAs and circulating hepcidin identify a subset of infertile women and may constitute a target for therapy.