Elevated circulating micro-ribonucleic acid (miRNA)-200b and miRNA-429 levels in anovulatory women

Elevated expression levels of serum circulating miRNA- 200b and miRNA-429 were observed in anovulatory women with polycystic ovary syndrome during the early follicular phase of the cycle compared with normal ovulating women.

Volume 107, Issue 1, Pages 269-275


Iris Eisenberg, Ph.D., Neta Nahmias, M.D., Michal Novoselsky Persky, M.D., Caryn Greenfield, M.Sc., Debra Goldman-Wohl, Ph.D., Arye Hurwitz, M.D., Ronit Haimov-Kochman, M.D., Simcha Yagel, M.D., Tal Imbar, M.D.



To study the role of micro-RNA (miRNA)-200b and miRNA-429 in human ovulation and to measure their expression levels in ovulatory and anovulatory patients.


Micro-RNA-200b and miRNA-429 expression analysis in human serum and granulosa cells at different phases of the ovulation cycle in normal cycling women and women undergoing assisted reproductive technology cycles.


University-affiliated hospital and academic research laboratory.


Forty women (7 normally ovulating, 15 normally ovulating with pure male infertility factor, and 18 with polycystic ovary syndrome) were included in this study.



Main Outcome Measure(s)

The expression profile of circulating miRNAs and granulosa cells was assessed by means of real-time quantitative reverse transcription–polymerase chain reaction analysis.


We identified miRNA-200b and miRNA-429 in the sera of all women tested. These miRNA expression levels were elevated during the early follicular phase of the cycle compared with serum levels during the early luteal phase. Anovulatory women, diagnosed with polycystic ovary syndrome, expressed significantly higher levels of miRNA-200b and miRNA-429 compared with spontaneously ovulating women. Ovulation induction with exogenous gonadotropins during an IVF cycle reduced these levels to the levels measured in normal ovulating women.


Our findings suggest an involvement of miRNA-200b and miRNA-429 in the pituitary regulation of human ovulation. Although it is unclear whether this altered miRNA expression profile is a cause or a result of anovulation, the levels of these molecules in the serum of anovulatory women may serve as serum biomarkers for the ovulation process.

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Go to the profile of Amanda Kallen
almost 6 years ago
Thanks to the authors for this interesting work. While the data certainly suggests that there may be a link between miR200b/miR249 expression and pituitary regulation of ovarian function, I agree that future studies need to further tease out the role of miR200b and 249 in glucose metabolism and insulin resistance, as these are potential confounders. The difference in BMI between control and PCOS groups is notable. Would like to know more about the characteristics of the PCOS population studied - did they have insulin resistance? Hyperandrogenemia? is there any data that these miRs have roles in androgen metabolism? Nevertheless the research is very intriguing and I look forward to the future work as well.