HOXA-10 and E-cadherin expression in the endometrium of women with recurrent implantation failure and recurrent miscarriage
Endometrial HOXA-10 and E-cadherin expressions are reduced in women with recurrent implantation failure and recurrent miscarriage compared with fertile control women, suggesting that they play a role in the implantation process.
Volume 107, Issue 1, Pages 136-143
Yihua Yang, Ph.D., Xiaoyan Chen, M.Phil., Sotirios H. Saravelos, M.B.B.S., Yingyu Liu, M.Phil, Jin Huang, Ph.D., Jiamiao Zhang, M.Phil., Tin Chiu Li, M.D., Ph.D.
To compare the expression of HOXA-10 and E-cadherin in the endometrium of women with recurrent implantation failure (RIF), women with recurrent miscarriage (RM), and women with proven fertility (normal control; NC).
Observational cohort study.
University assisted reproductive unit.
Fifty women were recruited: 18 NC, 12 unexplained RIF, and 20 RM.
Main Outcome Measure(s)
Endometrial biopsy was precisely timed 7 days after LH surge. The expression of HOXA-10 and E-cadherin were examined by means of immunohistochemistry. H-Scores of staining intensity in the glandular epithelium and stroma were measured.
HOXA-10 signal was mainly localized in the nuclei of stroma cells and the cytoplasm of glandular epithelium cells. E-Cadherin signal was found only in the cytoplasm of glandular epithelium cells. The HOXA-10 H-scores in the RIF group and the RM group were significantly lower than in the control group in both the glandular epithelium and stroma. The E-cadherin H-scores in the RM group were also significantly lower than in the control group. Interestingly, there was a positive correlation between HOXA-10 and E-cadherin H-scores in all of the women examined.
There is a positive correlation between levels of HOXA-10 and E-cadherin expression in the endometrium, both of which are significantly reduced in women with RIF and RM compared with fertile control women. The findings suggest a potential role of HOXA-10 and E-cadherin in the implantation processes and altered expression in women with reproductive failure.