Immune-inflammation gene signatures in endometriosis patients

With the use of the novel Nanostring platform, we determined the inflammatory and immune molecular signatures of endometriotic tissues and provide information on the potential interplay of dysfunctional inflammation and immunity in the pathogenesis of endometriosis.

Like Comment

Volume 106, Issue 6, Pages 1420-1431

Authors:

Soo Hyun Ahn, M.Sc., Kasra Khalaj, M.Sc., Steven L. Young, M.D., Ph.D., Bruce A. Lessey, M.D., Ph.D., Madhuri Koti, D.V.M., Ph.D., Chandrakant Tayade, D.V.M., Ph.D.

Abstract:

Objective

To determine if the molecular profiles of endometriotic lesions contain informative measures of inflammation and immune dysfunction that may contribute to better understanding of the interplay between immune dysfunction and inflammation and their contribution to endometriosis pathogenesis.

Design

Immune and inflammation transcriptomic analysis with the use of the Nanostring nCounter GX Human Immunology V2 platform (579 human immune and inflammation–related genes and 15 housekeeping genes).

Setting

Academic university and teaching hospital.

Intervention(s)

None.

Patient(s)

Stage III–IV endometriosis patients with infertility (n = 8) and fertile disease-free control women undergoing tubal ligation (n = 8). Menstrual stage was matched to secretory phase in all participants.

Main Outcome Measure(s)

Immune and inflammation transcriptomics quantification from ectopic endometriotic lesions and matched eutopic endometrium from patients. Endometria of fertile women served as control subjects.

Result(s)

Our results displayed endometriotic lesions as molecularly distinct entities compared with eutopic endometrium and endometrium of control samples; 396 out of 579 screened immune and inflammation–related genes were significantly different in ectopic tissues compared with control endometrium. Most importantly, eutopic endometrium of the patients displayed a unique molecular profile compared with the control endometrium (91/579 genes were significantly different), particularly of genes involved in regulation of cell apoptosis and decidualization.

Conclusion(s)

We characterize differential expression of immune-inflammation genes in endometriosis patients, and show molecular distinction of eutopic endometrium of patients compared with control fertile women.


Read the full text here.

Fertility and Sterility

Editorial Office, American Society for Reproductive Medicine

Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

No comments yet.