Cumulus cells have longer telomeres than leukocytes in reproductive-age women

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Authors:

Evelin E. Lara-Molina, M.D., Jason M. Franasiak, M.D., Diego Marin, Ph.D., Xin Tao, Ph.D., H.C.L.D., Patricia Díaz-Gimeno, Ph.D., Mireia Florensa, M.S., Marta Martin, M.S., Emre Seli, M.D., Antonio Pellicer, M.D., Ph.D.

Abstract:

Objective

To investigate whether telomere length (TL) in granulosa cells (GC) or cumulus cells (CC) correlates with TL in leukocytes (L).

Design

Prospective noninterventional study.

Setting

Private assisted reproductive technology center.

Patient(s)

Thirty-five egg donors were included in the study.

Interventions(s)

None.

Main Outcome Measure(s)

Average relative leukocyte telomere length (LTL), cumulus cell telomere length (CCTL), and granulosa cell telomere length (GCTL) measurements from each study subject.

Result(s)

Participants had a mean age of 25.43 ± 4.57 years, antimüllerian hormone level of 1.90 ± 0.92 ng/mL, antral follicle count of 23.29 ± 5.11, and the mean number of mature oocytes retrieved was 23.29 ± 9.13. No significant association between these variables and GCTL, CCTL, or LTL was found. In addition, no correlation was observed between TL measurements of L vs. CC, L vs. GC, or CC vs. GC. Interestingly, CCTL was significantly higher than LTL (1.54-fold), although no significant differences were found between GCTL vs. CCTL or GCTL vs. LTL.

Conclusion(s)

CC from mature follicles have significantly longer telomeres than L, suggesting that the follicular environment could possess different mechanisms to cope against telomere shortening compared with other somatic tissues. Furthermore, these data do not support the utility of telomere DNA measurement in L as an estimate of TL in follicular cells.


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Fertility and Sterility® is an international journal for obstetricians, gynecologists, reproductive endocrinologists, urologists, basic scientists and others who treat and investigate problems of infertility and human reproductive disorders. The journal publishes juried original scientific articles in clinical and laboratory research relevant to reproductive endocrinology, urology, andrology, physiology, immunology, genetics, contraception, and menopause. Fertility and Sterility® encourages and supports meaningful basic and clinical research, and facilitates and promotes excellence in professional education, in the field of reproductive medicine.

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