April 2012

All the papers from the April 2012 issue of Fertility and Sterility.

Development and validation of an accurate quantitative real-time polymerase chain reaction–based assay for human blastocyst comprehensive chromosomal aneuploidy screening

A 4-hour quantitative real-time polymerase chain reaction method for preimplantation comprehensive 24-chromosome aneuploidy screening was developed and validated. Results demonstrated 97.6% accuracy on cell lines and 98.6% consistency with microarray-based blastocyst diagnoses.

Elective single-embryo transfer

The optimal outcome of in vitro fertilization is the birth of a healthy singleton. Elective single-embryo transfer should be offered to good-prognosis patients. Elective single-embryo transfer utilization should increase with improved awareness and embryology laboratory advances.

Comprehensive chromosome screening is highly predictive of the reproductive potential of human embryos: a prospective, blinded, nonselection study

This prospective, blinded, nonselection study is the first to determine the negative and positive predictive values of aneuploidy screening for clinical outcome. Results demonstrate that this CCS method can be safely used to discard embryos because they rarely possess significant reproductive potential.

Chemokine CCL2 enhances survival and invasiveness of endometrial stromal cells in an autocrine manner by activating Akt and MAPK/Erk1/2 signal pathway

The ESC-derived CCL2 enhances the expression of proliferating cell nuclear antigen, survivin, and matrix metalloproteinase-2, decreases tissue inhibitor of metalloproteinase 1/2 expression, and promotes the growth and invasiveness of ESCs in an autocrine manner through the Akt and MAPK/Erk1/2 signal pathway.