Development and characterization of an endometrial tissue culture model for study of early implantation events
An improved endometrial tissue culture system was developed. The cultured tissue responded to steroids, allowed leukemia inhibitory factor–dependent blastocyst attachment, and underwent decidualized reaction in vitro.
Tian-Min Ye, M.D., Ph.D., Ronald T. K.Pang, Ph.D., Carmen O. N. Leung, M.Med.Sc., Weimin Liu, Ph.D., William S. B.Yeung, Ph.D.
Vol 98, Issue 6, Pages 1581-1589
To improve and characterize an endometrial tissue culture model.
Experimental study of the characteristics of mouse endometrial tissue cultured on amniotic membrane matrix.
University research laboratory.
Sexually mature female ICR mice.
Histological examination of the cultured endometrial tissues. The attachment rates of the cultured tissues to implantation blastocysts under various conditions were determined.
Main Outcome measures:
Morphometric analysis of the cultured tissues. Blastocyst attachment rate and expression of decidualization markers cylcooxygenase-2, connexin 43, and peroxisome proliferator-activated receptor δ.
Endometrial tissues could be grown on AMM for 3 days with morphometric parameters similar to the in vivo pseudopregnant control. The cultured tissues responded to the surrounding steroid environment. Morphometric assessment indicated medium containing 63.5 nmol/L progesterone and 0.9 nmol/L estradiol provided the best support. The condition allowed attachment of about 60% of the cocultured blastocysts. A small percentage of the attached blastocyst started to penetrate the luminal epithelium within 28 hours. The attachment rate was significantly reduced with prior treatment of the cultured endometrium with anti-leukaemia inhibitory factor antibody. The attached blastocyst induced decidualization around the attachment site.
The model is useful for the study on implantation in the mouse.
Read the full text at: http://www.fertstert.org/article/S0015-0282(12)02065-1/fulltext