Standardization of laparoscopic pelvic examination
Standardization of laparoscopic pelvic examination will make lesions in anatomically challenging locations less likely to miss.
April 2013
All the papers from the April 2013 issue of Fertility and Sterility.
Different levels of leptin regulate different target enzymes involved in progesterone synthesis
Leptin is able to regulate the expression of different target enzymes throughout the P synthesis, such as P450scc and 3bHSD, in a dose-dependent manner.
Follicle microenvironment associated alterations in gene expression in the mouse oocyte and its polar body
Changes in the follicle microenvironment lead to quantifiable differences in gene expression in individual oocytes and their polar bodies.
Cell membrane proteins from oviductal epithelial cell line protect human spermatozoa from oxidative damage
Cell membrane proteins from an oviductal epithelial cell line have protective effects on spermatozoa against oxidative stress.
Kisspeptin stimulates progesterone secretion via the Erk1/2 mitogen-activated protein kinase signaling pathway in rat luteal cells
Kisspeptin could augment progesterone secretion via stimulating key steroidogenic enzymes and phosphorylate Erk1/2 mitogen-activated protein kinase in cultured luteal cells.
Cryoprotectants up-regulate expression of mouse oocyte AQP7, which facilitates water diffusion during cryopreservation
Dimethylsulfoxide may up-regulate AQP7 expression in mouse oocytes by both osmolarity-dependent and osmolarity-independent pathways, and up-regulation of AQP7 facilitates water diffusion in mouse oocytes.
Feedback to professionals on patient-centered fertility care is insufficient for improvement: a mixed-method study
In audit and feedback methods to improve patientcenteredness in fertility care, the key issues are increasing professionals’ knowledge about their performance and their desire to make changes.
Training in empathic skills improves the patient-physician relationship during the first consultation in a fertility clinic
Training in empathic skills for physicians resulted in higher self-reported patient satisfaction regarding information quality, communication skills, time dedicated, and expertise in patients from different countries accessing infertility treatment.
In vitro fertilization for Orthodox Jewish couples: antagonist cycle modifications allowing for mikveh attendance before oocyte retrieval
A novel modification of gonadotropin-releasing hormone antagonist IVF protocols for postponement of retrieval until at least 7 days after cessation of menstruation allows for observance of ritual Jewish practices.
Preimplantation genetic screening using fluorescence in situ hybridization in patients with repetitive implantation failure and advanced maternal age: two randomized trials
Preimplantation genetic screening using fluorescence in situ hybridization improves live-birth rates in patients with repetitive implantation failure or advanced maternal age.
Uterine rudiments in patients with Mayer-Rokitansky-Küster-Hauser syndrome consist of typical uterine tissue types with predominantly basalis-like endometrium
Immunohistochemical analysis reveals all uterine tissue types in uterine rudiments of patients with MRKH syndrome. Endometrial components show reduced proliferative capacity and basalis-like differentiation.
Self-reported menopausal symptoms, coronary artery calcification, and carotid intima-media thickness in recently menopausal women screened for the Kronos early estrogen prevention study (KEEPS)
In recently menopausal women, self-reported climacteric symptoms do not correlate with subclinical atherosclerosis.
Evaluation of targeted next-generation sequencing–based preimplantation genetic diagnosis of monogenic disease
A methodology of next-generation sequencing was developed for preimplantation genetic diagnosis and found to be highly consistent with two independent methods of single-gene disorder screening.
Preimplantation genetic diagnosis outcomes and meiotic segregation analysis of robertsonian translocation carriers
The rate of alternate embryos can be affected by the sex of the robertsonian translocation carrier, and aneuploidy of chromosome 18 was significantly (P<.001) increased in 3:0-segregated or chaotic embryos.
Should we isolate human preantral follicles before or after cryopreservation of ovarian tissue?
Human preantral follicles can be successfully cryopreserved before or after isolation without impairing their ability to survive and grow in vitro.
Optimizing human oocyte cryopreservation for fertility preservation patients: should we mature then freeze or freeze then mature
Germinal vesicle and metaphase I oocytes should be matured in vitro before cryopreservation to optimize the reproductive potential of all retrieved oocytes.
MicroRNA miR-145 inhibits proliferation, invasiveness, and stem cell phenotype of an in vitro endometriosis model by targeting multiple cytoskeletal elements and pluripotency factors
MicroRNA miR-145 inhibits proliferation, invasiveness, and the stem cell phenotype of endometriotic cells by targeting multiple cytoskeletal elements and pluripotency factors, emerging as a novel target for therapeutic approaches.
Dopamine receptor D2 genotype (3438) is associated with moderate/severe endometriosis in infertile women in Brazil
The frequency of the dopamine receptor D2 polymorphism 2 was increased in subjects with peritoneal moderate/severe endometriosis.
Expression of the pluripotent transcription factor OCT4 promotes cell migration in endometriosis
OCT4 is significantly up-regulated in human ectopic endometriotic tissues. The OCT4 expression may contribute to the pathology of ectopic endometrial growth by stimulating the migration of endometrial cells.
Prevalence of infertility in the United States as estimated by the current duration approach and a traditional constructed approach
The prevalence of infertility in the United States was approximately twofold higher when estimated by a current duration approach vs. a traditional constructed measure (15.5% vs. 7.0%, respectively).
Treatment discontinuation in couples consulting for male infertility after failing to conceive
Of the 1,345 couples who consulted for male factor infertility, 407 patients discontinued treatment principally due to painfulness of treatment and its ineffectiveness. Of these patients, 20% finally succeeded in having a child.
Association of maternal and fetal MTHFR A1298C polymorphism with the risk of pregnancy loss: a study of an Indian population and a meta-analysi
The data of the present study clearly suggest that MTHFR A1298C polymorphism is a genetic risk factor for pregnancy loss, and it appears to be clinically important.
Antimüllerian hormone levels decrease in women using combined contraception independently of administration route
In young healthy, normal weight women, continuous use of combined contraception leads to a decrease in serum AMH levels independently of administration route.
Is the nuclear status of an embryo an independent factor to predict its ability to develop to term?
Blastomere multinucleation has a negative impact on live birth.
Couples dropping out of a reimbursed intrauterine insemination program: what is their prognostic profile and why do they drop out?
Dropping out of couples from fertility treatment is well known. An observational retrospective cohort study was performed to evaluate whether prognostic profiles differed between couples who drop out and couples that continue IUI.
Intracytoplasmic morphologically selected sperm injection versus intracytoplasmic sperm injection: a step toward a clinical algorithm
Our group experience demonstrates a clinical advantage for IMSI procedure in patients who failed in their first ICSI or for patients who shifted between ICSI and IMSI modalities.
Trophectoderm grade predicts outcomes of single blastocyst transfers
Trophectoderm grading, but not inner cell mass grading, significantly correlates with implantation and live birth for single-embryo transfers.
Consecutive gonadotropin-releasing hormone-antagonist in vitro fertilization cycles: does the elapsed time interval between successive treatments affect outcomes?
After IVF cycle failure, delaying consecutive IVF cycle start for two or more menstrual cycles is likely of no advantage compared with waiting only one endogenous menstrual cycle before proceeding.
A case report of an incidental finding of a 46,XX, SRY-negative male with masculine phenotype during standard fertility workup with review of the literature and proposed immediate and long-term management guidance
An incidental finding of a 46,XX SRY-negative phenotype was discovered during routine infertility investigations. We describe the case and propose clinical management guidance for the comprehensive management of this rare condition.
Does early cell death cause germ cell loss after intratesticular tissue grafting?
Spermatogonia-specific apoptosis does not explain the stem cell loss observed after intratesticular tissue grafting. Probably, hypoxia during the first days after transplantation induces degeneration of tubules in the center of the graft.
Seminal plasma adipokine levels are correlated with functional characteristics of spermatozoa
Obesity has an impact on semen quality, and adipokines appear to influence semen parameters potentially in conjunction with body mass index.
Altered microRNA expression profiles of human spermatozoa in patients with different spermatogenic impairments
Microarray and real-time polymerase chain reaction (PCR) studies reveal that microRNAs are differentially expressed in normal versus spermatogenically impaired males and have therapeutic potential for the development of biomarkers for male infertility.
The apoptotic pathway in fertile and subfertile men: a case-control and prospective study to examine the impact of merocyanine 540 bodies on ejaculated spermatozoa
The presence of merocyanine 540 staining bodies does not affect quantification of early and late apoptotic biomarkers in spermatozoa from fertile and subfertile men.
Are sperm DNA fragmentation, hyperactivation, and hyaluronan-binding ability predictive for fertilization and embryo development in in vitro fertilization and intracytoplasmic sperm injection?
Because DNA fragmentation, hyaluronan-binding ability, and induced hyperactivity of spermatozoa correlate with the fertilization rate after in vitro fertilization, blastocyst formation can be predicted by the sperm DNA fragmentation rate
Scoring human sperm morphology using Testsimplets and Diff-Quik slides
For assessing sperm morphology, Testsimplets gives a statistically significantly lower number of normal forms, so its clinical application is not recommended over Diff-Quik, the quickest recommended technique.
Two-dimensional differential in-gel electrophoresis–based proteomics of male gametes in relation to oxidative stress
Proteomic analysis shows that differences in protein expression in spermatozoa producing higher levels of ROS may play a role in the pathophysiology of male infertility.
Mystery of idiopathic male infertility: is oxidative stress an actual risk?
Sperm ROS formation and DNA damage as well as seminal plasma oxidative stress parameters were investigated in idiopathic infertile men. Intensive oxidative stress may have caused sperm dysfunction.
Standardized photographic surgical documentation—a picture is worth a thousand words
Reflections on “Standardization of laparoscopic pelvic examination” by Bedaiwy et al.
Interaction between oviductal epithelial cells and spermatozoa underlies a systems biology approach to treating infertility
Reflections on “Cell membrane proteins from oviductal epithelial cell line protect human spermatozoa from oxidative damage” by Chiu et al.
One swallow does not make a summer
Reflections on “Preimplantation genetic screening using fluorescence in situ hybridization in patients with repetitive implantation failure and advanced maternal age: two randomized trials” by Rubio et al.