Vasovasostomy: a step by step surgical technique video
This video presentation demonstrates the step-by-step microsurgical approach to the twolayered vasovasostomy technique by an experienced urologic microsurgeon.
March 2014
All the papers from the March 2014 issue of Fertility and Sterility.
Complete method to obtain culture and transfer mouse blastocysts nonsurgically to study implantation and development
This step-by-step video explains how to obtain, culture, and transfer blastocysts in order to study implantation and development using a new non-surgical method.
Role for the endometrial epithelial protein MFG E8 and its receptor integrin αvβ3 in human implantation Results of an in vitro trophoblast attachment study using established human cell lines
MFG-E8 and its receptor integrin avb3 are involved in the attachment of trophoblasts to the endometrial epithelium.
Obese fathers metabolic state adiposity and reproductive capacity indicate a sons reproductive health
Diet and exercise interventions in obese founder males improve the reproductive health of male offspring, with a father’s metabolic and reproductive status predicting his son’s reproductive health.
Digital multiplexed mRNA analysis of functionally important genes in single human oocytes and correlation of changes in transcript levels with oocyte protein expression
Measurement of transcript levels in single human oocytes with the use of Nanostring and correlation with protein expression identified biologically meaningful oocyte-to-oocyte heterogeneity in functionally important regulatory pathways.
Validation of the power model of ovarian nongrowing follicle depletion associated with aging in women
In an independent set of ovarian nongrowing follicle counts, the power model of ovarian nongrowing follicle depletion was determined to be an excellent fit to the observed counts.
Enzyme linked immunosorbent assay measurements of antimüllerian hormone in human blood are a composite of the uncleaved and bioactive cleaved forms of AMH
Enzyme-linked immunosorbent assay measurement of antim€ullerian hormone (AMH) is a composite of inactive precursor and receptor-competent forms of AMH, which may confound its use for clinical assessment of ovarian function.
Hyperandrogenemia is implicated in both the metabolic and reproductive morbidities of polycystic ovary syndrome
A factor analysis identified multiple factors that are responsible for the abnormalities associated with PCOS. Hyperandrogenemia was a common underlying feature of the metabolic and reproductive abnormalities in women with PCOS.
The type 2 deiodinase Thr92Ala polymorphism is associated with disrupted placental activity but not with dysglycemia or adverse gestational outcomes A genetic association study
Patients with type 2 deiodinase (D2) Ala92Ala genotype, associated with an increased risk for diabetes, have decreased placental D2 activity that is not associated with dysglycemia or adverse gestational outcomes.
Association of five common polymorphisms in the plasminogen activator inhibitor 1 gene with primary ovarian insufficiency
PAI-1 9785GA+AA, 844A/9785A, 4G/9785A, and 9785A/11053Gmay contribute to prevalence of primary ovarian insufficiency (POI) in Korean women.
Chromosome size, morphology, and gene density determine bivalent positioning in metaphase I human spermatocytes
This study demonstrates that distribution in metaphase I is nonrandom and influenced by chromosome size, gene density, and acrocentric chromosome morphology. Results support that some features defining chromosome territories are maintained during meiosis.
Neoadjuvant intraarterial chemotherapy followed by total laparoscopic radical trachelectomy in stage IB1 cervical cancer
Neoadjuvant intra-arterial chemotherapy followed by total LRT may become a useful option for women with cervical cancer with a tumor size larger than 2 cm who wish to preserve fertility.
Outcomes of operative sperm retrieval strategies for fertility preservation among males scheduled to undergo cancer treatment
Electroejaculation and testicular sperm extraction led to successful sperm retrieval in 60% of adolescents and teenagers and 59% of adult men seeking fertility preservation before cancer therapy.
Confocal laser scanning microscopy analysis of bioenergetic potential and oxidative stress in fresh and frozen thawed human ovarian tissue from oncologic patients
In slow-frozen/rapid-thawed ovarian tissue from cancer patients, a bioenergy/oxidative stress assessment based on confocal laser scanning microscopy allows identification of functionally preserved tissue.
Prognostics factors of oncologic and reproductive outcomes in fertility sparing management of endometrial atypical hyperplasia and adenocarcinoma Systematic review and meta analysis
In this meta-analysis including patients who underwent uterine preservation for endometrial neoplasia, oncologic and reproductive outcomes were not impaired in older patients with previous infertility or obesity.
Improving ovarian tissue cryopreservation for oncologic patients Slow freezing versus vitrification effect of different procedures and devices
Vitrification of ovarian tissue employing ethylene glycol and dimethyl sulfoxide in metallic grids preserves morphology of follicles and stroma, and maintains proliferation, viability, and vascularization status similarly to fresh tissue.
Knowledge attitudes and practices regarding conception and fertility A population based survey among reproductive age United States women
Knowledge regarding ovulation, fertility, and conception is limited in key areas among a sample of reproductive-age US women. Future initiatives should prioritize improved provider engagement and accurate information dissemination in Web-based venues.
Female dietary antioxidant intake and time to pregnancy among couples treated for unexplained infertility
Antioxidant intake and time to pregnancy (TTP) among couples treated for infertility was examined. Increased b-carotene, vitamin C, or vitamin E intakes are associated with shorter TTP in specific subgroups.
Pathophysiologic explanation for bladder retention in patients after laparosopic surgery for deeply infiltrating rectovaginal and or parametric endometriosis
The most frequent etiology for bladder retention after surgery for deeply infiltrating endometriosis seems to be chronic bladder overdistention by insidious bladder overdistention.
Ileocecal endometriosis Clinical and pathogenetic implications of an underdiagnosed condition
The radical surgical resection of associated ileocecal andrectovaginal endometriosis reduces pelvicpain, constipation, and dyschezia during a long-term follow-up.
Overxpression of lysine specific demethylase 1 in ovarian endometriomas and its inhibition reduces cellular proliferation cell cycle progression and invasiveness
Lysine-specific demethylase 1 (LSD1) is overexpressed in endometriomas; treatment of endometriotic stromal cells with tranylcypromine, an LSD1 inhibitor, significantly reduces cellular proliferation, cell cycle progression, and invasiveness.
GSTO1 uncommon genetic variants are associated with recurrent miscarriage risk
GSTO1, which is involved in the biotransformation process of arsenic, a well-known risk factor for pregnancy loss, is associated with recurrent miscarriage risk.
Long term risk of ectopic pregnancy varies by method of tubal sterilization A whole population study
Ectopic pregnancy can occur after tubal sterilization, with the greatest risk for women sterilized before the age of 28 years or with laparoscopic electrodestruction or partial salpingectomy.
Unilateral oophorectomy results in compensatory follicular recruitment in the remaining ovary at time of ovarian stimulation for in vitro fertilization
Women with a prior unilateral oophorectomy have compensatory follicular recruitment in the remaining ovary, and right ovaries respond better than left ovaries after stimulation for IVF.
Effect of fibroids not distorting the endometrial cavity on the outcome of in vitro fertilization treatment A retrospective cohort study
A retrospective cohort study demonstrates that intramural fibroids with diameter larger than 2.85 cm have a negative effect on in vitro fertilization/intracytoplasmic sperm injection delivery rates.
A randomized clinical trial of endometrial perfusion with granulocyte colony stimulating factor in in vitro fertilization cycles Impact on endometrial thickness and clinical pregnancy rates
Granulocyte colony-stimulating factor neither affects endometrial thickness nor pregnancy rates in normal in vitro fertilization patients.
Comparison of complication rates and pain scores after transvaginal ultrasound guided oocyte pickup procedures for in vitro maturation and in vitro fertilization cycles
Complication types and rates seem similar after transvaginal ultrasound–guided oocyte collection in in vitro maturation and conventionally stimulated in vitro fertilization cycles.
Increasing the probability of selecting chromosomally normal embryos by time lapse morphokinetics analysis
A retrospective study demonstrates differences in the kinetics between chromosomally normal and abnormal embryos, providing a selection tool that increases the probability of selecting normal embryos through a noninvasive method.
Rescue intracytoplasmic sperm injection A systematic review
A literature search using the term “rescue ICSI” was performed. Thirty-eight studies were included, describing 1,863 patients and 194 offspring. Pregnancy rates and procedure safety are discussed.
Factors associated with monozygosity in assisted reproductive technology pregnancies and the risk of recurrence using linked cycles
The risk of monozygosity was higher with fresh day 5–6 embryos, donor oocytes, gonadotropin-releasing hormone agonist suppression, lower follicle-stimulating hormone doses, and assisted hatching (particularly day 2–3 embryos).
Progesterone level at oocyte retrieval predicts in vitro fertilization success in a short antagonist protocol A prospective cohort study
Progesterone (P) level at oocyte retrieval was inversely associated with pregnancy rates: as P levels increased, pregnancy rates decreased. The odds ratio for nonpregnancy was clinically significant when P was 12 ng/mL.
Cabergoline for the prevention of ovarian hyperstimulation syndrome systematic review and meta analysis of randomized controlled trials
Cabergoline reduces the occurrence of moderate-severe ovarian hyperstimulation syndrome and probably does not have a clinically relevant impact on clinical pregnancy or on the number of retrieved oocytes.
The nature of aneuploidy with increasing age of the female partner a review of 15169 consecutive trophectoderm biopsies evaluated with comprehensive chromosomal screening
The lowest aneuploidy risk is between ages 26–30, with higher prevalences in younger and older women, and 67% involve a single chromosome,with the ratio of trisomies/monosomies approximating 1.
Age does not adversely affect sperm retrieval in men undergoing microdissection testicular sperm extraction
Sperm retrieval in men undergoing microdissection testicular sperm extraction (micro-TESE) was not negatively affected by age. Men 50 years old who underwent successful micro-TESE were more likely to have larger testes.
Sperm selected by both birefringence and motile sperm organelle morphology examination have reduced deoxyribonucleic acid fragmentation
Birefringence and MSOME are effective to select sperm with improved nuclear status. We found that sperm selected by both these methods and no nuclear vacuoles have significantly lower DNA fragmentation.
Thawed human sperm quality is influenced by the volume of the cryopreserved specimen
Better quality thawed human sperm was achieved after cryopreservation of high volumes compared with low volumes of specimens. Air volume in the straw had no influence on specimen quality.
Angled vas cutter for vasovasostomy Technique and results
The angled vas cutting forceps represents a modification of the straight cut technique and has the potential advantage of an increase in vasal surface area for reanastomosis and possible improvement of patency rates.
Using contemporary microdissection testicular sperm extraction techniques older men with nonobstructive azoospermia should not be deterred from becoming fathers
Reflections on “Age does not adversely affect sperm retrieval in men undergoing microdissection testicular sperm extraction,” by Ramasamy et al.
Female age related fertility decline
Education and enhanced awareness of the effect of age on fertility is essential in counseling the patient who desires pregnancy.
Anonymity 2.0 Direct to consumer genetic testing and donor conception
Direct-to-consumer genetic testing services for genealogical or ancestry purposes increases the risk 31 of inadvertently finding out that someone was conceived using gamete donation as well as 32 discovering the identity of the gamete donor(s).