The germ cell transplant demonstrates that spermatogonial stem cells can engraft recipient seminiferous tubules and produce spermatogenesis. Here we describe the protocol for germ cell transplant by efferent duct injection.

This video demonstrates a technique for separating miscarriage tissue from maternal decidua to reduce maternal cell contamination for chromosome analysis.

Fertility after laparoscopic neosalpingostomy is largely dependent on the tubal status and should only be proposed for patients with tubal stage 1 or 2.

Conventional monopolar contact coagulation induces significantly more adhesions that are more vascularized than noncontact argon plasma in a randomized experimental rodent model.

RvD1 and 17(R)-RvD1 reduced cyst vascular permeability and associated vaginal hyperalgesia in rats with surgically-induced endometriosis suggesting that further investigation of therapeutic anti-inflammatory potentials of resolvins can be considered.

Vitrification can lead to a loss of deoxyribonucleic acid methylation of imprinted genes in blastocysts, possibly because of reduction of deoxyribonucleic acid methyltransferase abundance in mouse MII oocytes after vitrification.

During pregnancy, regulation of the expression of human chorionic gonadotropin beta subunit genes correlates with methylation of their promoters and TFAP2A expression.

The strong correlation of insulin resistance and oxidative stress at the visceral adipose tissue level suggests that local oxidative stress and abnormalities of insulin signaling in the adipose tissue play critical roles in polycystic ovary syndrome insulin resistance.

Several years after donation, a majority of 210 identity-release donors feel positive about future contact with an offspring, and their preferences should be taken into account by health-care services.

Patients with pelvic abscess treated with antibiotics alone are more likely to require surgical intervention than patients who also receive image guided drainage.

The study showed that genetic variants on the E-cadherin gene may be a potential molecular marker for the development of primary infertility in northern Chinese women with ovarian endometriosis.

In women with polycystic ovary syndrome, the variant allele of Fat mass and obesity associated (FTO) rs9939609 was associated with increased body mass index.

MED12 mutations inversely correlate with leiomyoma size; in this study where size bias is carefully controlled, over 80% of uterine leiomyomas display a MED12 mutation.

Molecular analyses of 58 patients with hypogonadotropic hypogonadism revealed the relatively minor contribution of known genetic defects, and identified submicroscopic genomic rearrangement and SOX3 and WDR11 mutations as rare etiologies.

This is the first study showing that repeated ejaculates from cancer patients did not show substantial variation in semen quality.

Compared with children born to fertile parents, no systematic differences were observed for body weight, height, body mass index, or head circumference at age 5 in children conceived after fertility treatment or in children born to subfertile parents.

Treatment with clomiphene citrate is associated with an increased risk of small for gestational age in children born after intrauterine insemination, whereas ovarian stimulation with follicle-stimulating hormone did not seem to increase the risk of adverse perinatal outcomes.

The study results suggest that physical activity is not deleterious to testicular function, as captured by semen quality parameters in this population of healthy young men in Spain.

In a cohort study, the incidence of hypertension increased in women after IVF pregnancies, suggesting that these women could have a propensity to develop cardiovascular disease later in life.

Increased mRNA expression of prokineticin 1 and leukemia inhibitory factor could be one of several abnormalities characterizing the endometrium of women with recurrent pregnancy loss.

Primary single-step surgical evacuation is adequate for treating cesarean section pregnancy in most patients with superficial implantation.

This study observed increased risk for fetal losses with variants of platelet-specific collagen receptor glycoprotein VI. In silico analysis revealed the influence of glycoprotein VI in biologic pathways, collagen binding, and gene-gene interactions.

Two nonsynonymous prokineticin receptor variants were significantly associated with idiopathic recurrent miscarriages, and endocrine gland–derived vascular endothelial growth factor variant has genetic interactions with two receptor genes that contribute to miscarriage risk.

Preconception fertility status affects the incidence of pregnancy-induced hypertension and delivery outcomes in women aged 40 years and older.

Numbers of oocytes retrieved and properties of day 3 embryos were predictive of blastocyst formation, implantation, and pregnancy. Patient age and oocyte yields independently impacted implantation rates and uterine receptivity.

Local endometrial injury does not seem to influence oocyte donation outcome.

In initial assisted reproduction cycles for which the only diagnosis is diminished ovarian reserve, the practices of assisted hatching and intracytoplasmic sperm injection are not associated with improved live birth rates.

Follicle rupture and the number of uterine contractions observed after intrauterine insemination are positively correlated with clinical pregnancy and live birth rates. A prediction model was built including these variables.

The presence of multinucleation in 2-cell stage embryos seems to have a negative impact on pregnancy outcome.

The aim of this work is to define what objective variables, obtained by image analysis, show better predictive value of embryo quality for implantation in women age <36 years. Smaller circular embryos with thinner zona pellucida are more likely to implant.

In 242 preimplantation genetic diagnoses, 242 intracytoplasmic sperm injections, and 733 spontaneous pregnancies, prematurity was more common after ICSI or PGD; intrauterine growth restriction was more common only after ICSI.

Obstetric and perinatal outcomes from singleton and multiple pregnancies obtained using vitrified oocytes revealed lack of clinically relevant differences when compared with those achieved with fresh oocytes.

This systematic review and meta-analysis showed that high sperm DNA fragmentation was related to lower pregnancy rates in IVF but not in ICSI cycles, whereas it was associated to higher miscarriage rates in both IVF and ICSI cycles.

Quantitative real-time polymerase chain reaction validation reveals that a set of five miRNAs deserves to be used as a diagnostic test along with other routine clinical tests, such as conventional semen and histologic analysis, to improve male infertility assessment and diagnosis.

Human semen air-dried before DNA fragmentation analysis provides statistically significantly closer results to fresh semen compared with snap-frozen semen and can be stored for up to 1 month.

The ROS declined and HO-1 increased after the thawing of human seminiferous tubules. Slow freezing is more effective than vitrification in terms of HO-1 up-regulation and ROS decrease.

Aberrant DNA methylation and abnormal DDR1 gene expression is associated with men with idiopathic nonobstructive azoospermia.

Reflections on “Genome-wide copy number analysis 1 and systematic mutation screening in 58 patients with hypogonadotropic hypogonadism” by Izumi et al.

This Committee Opinion concludes that donors be advised of the number of cycles/donations that a given oocyte donor may undergo. Although existing data cannot permit conclusive recommendations, a concern for the issues of the safety and well-being of oocyte donors warrants consideration. This document replaces the document of the same name, previously published in 2008 (Fertil Steril 2008; 90:S194–5).

This paper provides physicians with an overview of what personnel and processes constitute good laboratory practices in endocrinology, andrology, and embryology laboratories.